Planta

, Volume 191, Issue 3, pp 394–401

A sucrose-synthase gene of Vicia faba L.: Expression pattern in developing seeds in relation to starch synthesis and metabolic regulation

  • Ute Heim
  • Hans Weber
  • Helmut Bäumlein
  • Ulrich Wobus
Article

DOI: 10.1007/BF00195698

Cite this article as:
Heim, U., Weber, H., Bäumlein, H. et al. Planta (1993) 191: 394. doi:10.1007/BF00195698

Abstract

Copy-DNA clones encoding a single class of sucrose-synthase (SUCS; EC 2.4.1.13) subunit have been isolated and sequenced from a Vicia faba L. seed cotyledonary library. Southern analyses indicated the existence of only one gene. Transcript levels determined by Northern blot hybridisation steadily increased until the middle of development [25–35 days after flowering (DAF)] and declined thereafter. Sucrose levels approximately paralleled levels of SUCS mRNA. The activity of SUCS increased with decreasing fructose and glucose concentrations and peaked about 10 d later than mRNA levels. In-vitro culture experiments demonstrated that increasing the sucrose concentration leads to increased levels of SUCS mRNA. The SUCS mRNA was also synthesised in seed-coat tissue, but in lower amounts than in cotyledons and with a different developmental profile. The early peak level of SUCS mRNA (20 DAF) in seed coats coincided with the peak in the amount of sucrose and with a peak of transiently synthesised starch.

Key words

cDNA clone (sucrose synthase)Gene expression (sucrose synthase, seed development)Nucleotide sequence (sucrose synthase)Seed developmentSucrose synthase (cloning, expression, regulation)Vicia

Abbreviations

bp

basepairs

DAF

days after flowering

kb

kilobase

SUCS

sucrose synthase

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Ute Heim
    • 1
  • Hans Weber
    • 1
  • Helmut Bäumlein
    • 1
  • Ulrich Wobus
    • 1
  1. 1.Institut für Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany