Trees

, Volume 1, Issue 3, pp 191–194

An in vitro method for establishing mycorrhizae on coniferous tree seedlings

  • I. Kottke
  • M. Guttenberger
  • R. Hampp
  • F. Oberwinkler
Short Communication

DOI: 10.1007/BF00193562

Cite this article as:
Kottke, I., Guttenberger, M., Hampp, R. et al. Trees (1987) 1: 191. doi:10.1007/BF00193562

Summary

A method for in vitro synthesis of mycorrhizae on coniferous tree seedlings is described. Tree seedlings (Larix decidua Mill., Picea abies (L.) Karst, and Pinus sylvestris L.) and fungi Amanita muscaria (L. ex Fr.) Hooker, Piloderma croceum Erikss. et Hjorst., Pisolithus tinctorius (Pers.) Coker et Couch, and Suillus grevillei (Klotzsch) Singer were maintained under sterile conditions in petri dishes. Typical ectomycorrhizae were established within 2–3 weeks after inoculation and within 2 months after germination of seedlings. Eventually a high percentage of mycorrhizal root tips was obtained.

Key words

Basidiomycetes Coniferous trees Ectomycorrhiza In vitro culture Symbiosis 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • I. Kottke
    • 1
  • M. Guttenberger
    • 1
  • R. Hampp
    • 1
  • F. Oberwinkler
    • 1
  1. 1.Institut für Biologie I, Universität TübingenTübingenGermany