, Volume 37, Issue 3, pp 193-198

Isolation of a novel cDNA clone showing marked similarity to ME491/CD63 superfamily

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A novel cDNA clone, A15, was isolated by the differential screening of a cDNA library of an immature T cell line, HPB-ALL using radioactive cDNA probes from the mRNA of either HPB-ALL or peripheral blood lymphocytes. It hybridized to a single mRNA species of about 2.0 kilobases which is expressed in HPB-ALL cell line, but not in the PBL or a promyelocytic leukemia cell line, HL-60. The A15 gene codes for a protein of 244 amino acids which contains four potential transmembrane domains and four possible N-linked glycosylation sites. A computer-aided comparison showed a marked similarity to several other membrane proteins: CD9, CD37, CD53, TAPA-1, Sm23, CO-029, and ME491/CD63.

The nucleotide sequence data reported in this paper have been submitted to the GenBank, DDBJ, and EMBL nucleotide sequence databases and have been assigned the accession number D10653.