Abstract
The present report describes an initial characterization of actin from non-infected roots and symbiotic nodules of Phaseolus vulgaris L. cv. Negro damapa. Using anti-actin monoclonal antibodies, a 42-kDa polypeptide was identified in plant extracts. After two-dimensional SDS-PAGE analysis and Western blotting of actin fractions enriched using diethylaminoethyl-resin, the presence of one major isoform of actin in symbiotic nodules and two main isoforms in non-infected roots was revealed. Possible implications of this finding are discussed.
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Abbreviations
- MAB(s):
-
monoclonal antibody(ies)
- IEF:
-
isoelectric focusing
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We thank Jose Luis Zitlalpopoca for technical assistance. H.P. gratefully acknowledges financial support from CONACYT (Mexico) and the National Institute of Public Health of Mexico. This work was partially supported by grants DGAPA-UNAM IN208489 and CEE. C11 06228-M.
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Pérez, H.E., Sánchez, N., Vidali, L. et al. Actin isoforms in non-infected roots and symbiotic root nodules of Phaseolus vulgaris L.. Planta 193, 51–56 (1994). https://doi.org/10.1007/BF00191606
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DOI: https://doi.org/10.1007/BF00191606