Medical Microbiology and Immunology

, Volume 181, Issue 6, pp 339–349

Determination of IgG subclass antibodies to Pseudomonas aeruginosa outer membrane proteins in cystic fibrosis lung infection using immunoblotting and enzyme-linked immunosorbent assay

  • Tacjana Pressler
  • Gitte Kronborg
  • Geoffry H. Shand
  • Bendt Mansa
  • Niels Høiby
Original investigations

DOI: 10.1007/BF00191546

Cite this article as:
Pressler, T., Kronborg, G., Shand, G.H. et al. Med Microbiol Immunol (1992) 181: 339. doi:10.1007/BF00191546

Abstract

IgG subclass antibodies to Pseudomonas aemginosa outer membrane proteins (OMP) were investigated in serum from cystic fibrosis (CF) patients by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Fifteen patients (eight in good and seven in poor clinical condition) have been followed for an average of 13 years with multiple serum samples covering the preinfection, and early and late stages of chronic infection. Laser-scanning densitometry of photographs taken from immunoblots was used to quantify antibody level and compare with ELISA titres. The earliest anti-OMP antibodies to appear were of the IgG1 subclass. There was no significant difference in IgG subclass antibody levels to OMPs between patients in relatively good and poor clinical condition. Data presented indicate a high positive correlation among measurements of IgG subclass antibodies to P. aeruginosa OMPs using ELISA and immunoblotting.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Tacjana Pressler
    • 1
  • Gitte Kronborg
    • 2
  • Geoffry H. Shand
    • 3
  • Bendt Mansa
    • 4
  • Niels Høiby
    • 2
  1. 1.Cystic Fibrosis Center Department of PaediatricsRigshospitalCopenhagenDenmark
  2. 2.Cystic Fibrosis Center Department of Clinical MicrobiologyRigshospitalCopenhagenDenmark
  3. 3.Microbiology DepartmentDako Diagnostic LimitedElyUK
  4. 4.Department of Immunological R&DStatens SeruminstitutCopenhagenDenmark
  5. 5.Department of Clinical MicrobiologyRigshospitaletKøbenhavn ØDenmark

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