Immunogenetics

, Volume 42, Issue 3, pp 204–212

Identification and characterization of T-cell antigen receptor-related genes in phylogenetically diverse vertebrate species

  • Jonathan P. Rast
  • Robert N. Haire
  • Ronda T. Litman
  • Susan Pross
  • Gary W. Litman
Original Papers

DOI: 10.1007/BF00191226

Cite this article as:
Rast, J.P., Haire, R.N., Litman, R.T. et al. Immunogenetics (1995) 42: 204. doi:10.1007/BF00191226

Abstract

Characterization of the structure, multiplicity, organization, and cell lineage-specific expression of T-cell receptor (TCR) genes of nonmammalian vertebrate species is central to the understanding of the evolutionary origins of rearranging genes of the vertebrate immune system. We recently described a polymerase chain reaction (PCR) strategy that relies on short sequence similarities shared by nearly all vertebrate TCR and immunoglobulin (Ig) variable (V) regions and have used this approach to isolate a TCR beta (TCRB) homolog from a cartilaginous fish. Using these short PCR products as probes in spleen cDNA and genomic libraries, we were able to isolate a variety of unique TCR and TCR-like genes. Here we report the identification and characterization of a chicken TCR gamma (TCRG) homolog, apparent Xenopus and pufferfish TCR alpha (TCRA) homologs, and two horned shark TCR delta (TCRD)-like genes. In addition, we have identified what could be a novel representative of the Ig gene super-family in the pufferfish. This method of using short, minimally degenerate PCR primers should speed progress in the phylogenetic investigations of the TCR and related genes and lend important insights into both the origins and functions of these unique gene systems.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Jonathan P. Rast
    • 1
  • Robert N. Haire
    • 1
  • Ronda T. Litman
    • 1
  • Susan Pross
    • 2
  • Gary W. Litman
    • 1
  1. 1.All Children's HospitalUniversity of South FloridaSt. PetersburgUSA
  2. 2.University of South FloridaTampaUSA