Immunogenetics

, Volume 35, Issue 2, pp 112–116

DNA polymorphism of HLA class II genes in primary biliary cirrhosis

  • Niels Morling
  • Kim Dalhoff
  • Lars Fugger
  • Jørgen Georgsen
  • Bodil Jakobsen
  • Leo Ranek
  • Niels Ødum
  • Arne Svejgaard
Original Articles

DOI: 10.1007/BF00189520

Cite this article as:
Morling, N., Dalhoff, K., Fugger, L. et al. Immunogenetics (1992) 35: 112. doi:10.1007/BF00189520

Abstract

We investigated the DNA restriction fragment length polymorphism of the major histocompatibility complex class II genes: HLA-DRB,-DQA,-DQB, DPA,-DPB, the serologically defined HLA-A, B, C, DR antigens, and the primed lymphocyte typing defined HLA-DP antigens in 23 Danish patients with primary biliary cirrhosis (PBC) and in healthy Danes. The following genetic markers were found with increased frequencies in PBC: HLA-B8 (relative risk, RR=2.4, P<0.05, ‘corrected’ P>0.05), HLA-DR3 (RR=3.4, P<0.01, ‘corrected’ P<0.05), the DRB3*01/02/03 (DRw52) associated DRB Bgl II 9.1 kilobase (kb) fragment (RR= 2.9; P<0.05, ‘corrected’ P>0.05), the DQA1*0501 associated DQA Taq I4.8 kb fragment (RR=3.1; P<0.05, ‘corrected’ P>0.05), the DQB1*0201 (DQw2) associated DQB Hin dIII 11.5 kb fragment (RR=3.1; P<0.05, ‘corrected’ P>0.05). No DNA fragments specific for DRB1*0301 (DR3) could be identified. The frequencies in PBC of other genetic markers including DRw8, DRB1*08, HLA-DP antigens, DPA, and DPB genes did not differ significantly from those in controls. The associations between PBC and B8, DR3, DQA1*0501, and DQB*0201, which are frequently found together on the same haplotype, are at variance with recent reports on associations between PBC and Drw8. The discrepancy suggests that PBC is genetically heterogenous.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Niels Morling
    • 1
    • 3
  • Kim Dalhoff
    • 2
  • Lars Fugger
    • 1
  • Jørgen Georgsen
    • 1
  • Bodil Jakobsen
    • 1
  • Leo Ranek
    • 2
  • Niels Ødum
    • 1
  • Arne Svejgaard
    • 1
  1. 1.Tissue Typing LaboratoryCopenhagenDenmark
  2. 2.Medical Department AState University Hospital (Rigshospitalet)CopenhagenDenmark
  3. 3.Institute of Forensic GeneticsUniversity of CopenhagenCopenhagenDenmark
  4. 4.Institute of Forensic GeneticsUniversity of CopenhagenCopenhagen ØDenmark