Applied Microbiology and Biotechnology

, Volume 35, Issue 2, pp 231–232

(1,4)-β-d-Arabinoxylan arabinofuranohydrolase: a novel enzyme in the bioconversion of arabinoxylan

Authors

  • F. J. M. Kormelink
    • Department of Food ScienceAgricultural University
  • M. J. F. Searle-Van Leeuwen
    • Department of Food ScienceAgricultural University
  • T. M. Wood
    • The Rowett Research Institute
  • A. G. J. Voragen
    • Department of Food ScienceAgricultural University
Short Contribution

DOI: 10.1007/BF00184692

Cite this article as:
Kormelink, F.J.M., Searle-Van Leeuwen, M.J.F., Wood, T.M. et al. Appl Microbiol Biotechnol (1991) 35: 231. doi:10.1007/BF00184692

Summary

An enzyme able to hydrolyse the terminal non-reducing α-l-arabinofuranoside residues from arabinoxylans only has been found. This enzyme was unable to split arabinofuranosyl linkages in a range of other arabinofuranosyl-containing substrates. Analysis of reaction mixtures of arabinoxylan with this enzyme did not show a shift in the molecular weight distribution of the arabinoxylan, even after 24 h of incubation. Only monomeric arabinose was released. 1H-Nuclear magnetic resonance studies of arabinoxylan treated with this enzyme, described as (1,4)-β-d-arabinoxylan arabinofuranohydrolase, indicated a specificity towards the single-substituted xylose in arabinoxylan.

Copyright information

© Springer-Verlag 1991