Original Paper

Immunogenetics

, Volume 42, Issue 5, pp 315-322

First online:

Cloning and genomic characterization of LST1: a new gene in the human TNF region

  • Ingrid HolzingerAffiliated withUniversity Hospital for Internal Medicine, University of Innsbruck
  • , Annegret de BaeyAffiliated withInstitute for Anthropology and Human Genetics, University of Munich
  • , Gerald MesserAffiliated withDepartment of Dermatology, University of Munich
  • , Gerold KickAffiliated withDepartment of Dermatology, University of Munich
  • , Heinz ZwierzinaAffiliated withUniversity Hospital for Internal Medicine, University of Innsbruck
  • , Elizabeth H. WeissAffiliated withInstitute for Anthropology and Human Genetics, University of Munich

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The leucocyte specific transcript — 1 (LST1) represents the human homolog of the mouse B144 transcript, encoded within the tumor necrosis factor (TNF) region of the human major histocompatibility complex class III interval. The gene is localized about 4 kilobases upstream of the lymphotoxin ß gene. It spans a polymorphic genomic region encompassing the microsatellites TNFd and TNFe in intron 3 and a polymorphic Pvu II restriction site 260 base pairs downstream of the polyadenylation signal. Isolation of a full-length cDNA clone revealed that LST1 codes for IFN-γ-inducible 800 nt transcripts, which are present in lymphoid tissues, T cells, macrophages, and histiocyte cell lines. The cDNA contains three long open reading frames (ORF) with the most likely ORF encoding a transmembrane protein. Its close linkage to the TNF genes and pattern of expression point toward a possible role for LST1 in the immune response.