Journal of Molecular Evolution

, Volume 38, Issue 1, pp 18–27

Amplification of the ancient murine Lx family of long interspersed repeated DNA occurred during the murine radiation

  • Anthony V. Furano
  • Bruce E. Hayward
  • Pascale Chevret
  • François Catzeflis
  • Karen Usdin
Article

DOI: 10.1007/BF00175491

Cite this article as:
Furano, A.V., Hayward, B.E., Chevret, P. et al. J Mol Evol (1994) 38: 18. doi:10.1007/BF00175491

Abstract

We identified and characterized the relics of an ancient rodent Ll family, referred to as Lx, which was extensively amplified at the time of the murine radiation about 12 million years ago, and which we showed was ancestral to the modern L1 families in rat and mouse. Here we have extended our analysis of the Lx amplification by examining more murine and nonmurine species for Lx sequences using both blot hybridization and the polymerase chain reaction for a total of 36 species. In addition we have determined the relative copy number and sequence divergence, or age, of Lx elements in representative murine genera. Our results show that while Lx sequences are confined to murine genera, the extent of the amplification was different in the different murine lineages, indicating that the amplification of Lx did not precede, but was coincident with, the murine radiation. The implications of our findings for the evolutionary dynamics of L1 families and the utility of ancestral amplification events for systematics are discussed.

Key Words

L1 familyLINE familyMolecular synapomorphyAncient L1 familyRodent systematics

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Anthony V. Furano
    • 1
  • Bruce E. Hayward
    • 1
  • Pascale Chevret
    • 2
  • François Catzeflis
    • 2
  • Karen Usdin
    • 1
  1. 1.Section on Genomic Structure and Function, Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaUSA
  2. 2.Institut des Sciences de l'EvolutionUniversité Montpellier IIMontpellierFrance