Journal of Biomolecular NMR

, Volume 4, Issue 2, pp 301–306

A general enhancement scheme in heteronuclear multidimensional NMR employing pulsed field gradients

Authors

  • J. Schleucher
    • Institut für Organische ChemieUniversität Frankfurt
  • M. Schwendinger
    • Institut für Organische ChemieUniversität Frankfurt
  • M. Sattler
    • Institut für Organische ChemieUniversität Frankfurt
  • P. Schmidt
    • Institut für Organische ChemieUniversität Frankfurt
  • O. Schedletzky
    • Institut für Organische ChemieUniversität Frankfurt
  • S. J. Glaser
    • Institut für Organische ChemieUniversität Frankfurt
  • O. W. Sørensen
    • Novo Nordisk A/S
  • C. Griesinger
    • Institut für Organische ChemieUniversität Frankfurt
Short Communication

DOI: 10.1007/BF00175254

Cite this article as:
Schleucher, J., Schwendinger, M., Sattler, M. et al. J Biomol NMR (1994) 4: 301. doi:10.1007/BF00175254

Summary

General pulse sequence elements that achieve sensitivity-enhanced coherence transfer from a heteronucleus to protons of arbitrary multiplicity are introduced. The building blocks are derived from the sensitivity-enhancement scheme introduced by Cavanagh et al. ((1991) J. Magn. Reson., 91, 429–436), which was used in conjunction with gradient coherence selection by Kay et al. ((1992) J. Am. Chem. Soc., 114, 10663–10665), as well as from a multiple-pulse sequence effecting a heteronuclear planar coupling Hamiltonian. The building blocks are incorporated into heteronuclear correlation experiments, in conjunction with coherence selection by the formation of a heteronuclear gradient echo. This allows for efficient water suppression without the need for water presaturation. The methods are demonstrated in HSQC-type experiments on a sample of a decapeptide in H2O. The novel pulse sequence elements can be incorporated into multidimensional experiments.

Keywords

Coherence transferSensitivity enhancementB0-gradientsHeteronuclear NMRPlanar TOCSYHeteronuclear Hartmann-Hahn spectroscopyHSQCSolvent suppression

Copyright information

© ESCOM Science Publishers B.V 1994