Applied Microbiology and Biotechnology

, Volume 40, Issue 5, pp 638–643

Production of purified alginates suitable for use in immunoisolated transplantation

  • Gerd Klöck
  • Hermann Frank
  • Roland Houben
  • Tobias Zekorn
  • Andrea Horcher
  • Ulrike Siebers
  • Manfred Wöhrle
  • Konrad Federlin
  • Ulrich Zimmermann
Biotechnology

DOI: 10.1007/BF00173321

Cite this article as:
Klöck, G., Frank, H., Houben, R. et al. Appl Microbiol Biotechnol (1994) 40: 638. doi:10.1007/BF00173321

Abstract

Alginate is used as a matrix for immunoisolation of cells and tissues in vivo. We have demonstrated previously that commercial alginates contain various fractions of mitogenic impurities and that they can be removed by free flow electrophoresis. The use of purified material is a necessity in order to reveal the parameters that control biocompatibility of the implanted material (such as stability, size, surface charge and curvature, etc.). In this study, we present a protocol for the chemical purification of alginates on a large-scale. Beads made from alginates purified by this multi-step chemical extraction procedure did not induce a significant foreign body reaction when implanted for 3 weeks either intraperitoneally or beneath the kidney capsule of Lewis or non-diabetic BB/Gi rats.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Gerd Klöck
    • 1
  • Hermann Frank
    • 1
  • Roland Houben
    • 1
  • Tobias Zekorn
    • 2
  • Andrea Horcher
    • 2
  • Ulrike Siebers
    • 2
  • Manfred Wöhrle
    • 2
  • Konrad Federlin
    • 2
  • Ulrich Zimmermann
    • 1
  1. 1.Lehrstuhl für Biotechnologie, Biozentrum — Am HublandUniversity of WürzburgWürzburgGermany
  2. 2.Medizinische Klinik III und PoliklinikUniversity of GiessenGiessenGermany