Applied Microbiology and Biotechnology

, Volume 33, Issue 5, pp 542–546

Molecular cloning, nucleotide sequence and expression in Escherichia coli of the β-cyclodextrin glycosyltransferase gene from Bacillus circulans strain no. 8

  • Lars Nitschke
  • Karin Heeger
  • Hans Bender
  • Georg E. Schulz
Applied Genetics and Regulation

DOI: 10.1007/BF00172548

Cite this article as:
Nitschke, L., Heeger, K., Bender, H. et al. Appl Microbiol Biotechnol (1990) 33: 542. doi:10.1007/BF00172548

Summary

The β-cyclodextrin glycosyltransferase (β-CGTase) gene was isolated from a λ-library prepared from Bacillus circulans strain no. 8. It was subcloned into plasmid pTZ and expressed by its endogenous regulatory sequences in Escherichia coli JM 103. The structural gene was sequenced and showed an open reading frame for a polypeptide of 718 amino acid residues. The recombinant β-CGTase had the same enzymatic properties as the extracellular CGTase (684 amino acid residues, corresponding to a mol. wt. of 74416) produced by B. circulans strain no. 8. The amino acid sequence showed the highest homology (74.6% identical amino acids) with the CGTase of B. circulans strain F-2, which had been erroneously described as an amylase. The homology with the enzyme from the alkalophilic Bacillus sp. strain no. 1011 was 71.4%. The amino acid sequence derived will be used for elucidating the three-dimensional structure of the enzyme.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Lars Nitschke
    • 1
  • Karin Heeger
    • 1
  • Hans Bender
    • 1
  • Georg E. Schulz
    • 1
  1. 1.Institut für Organische Chemie und BiochemieFreiburg im BreisgauFederal Republic of Germany
  2. 2.Max Planck Institut für ImmunbiologieFreiburg im BreisgauFederal Republic of Germany