Applied Microbiology and Biotechnology

, Volume 42, Issue 5, pp 658–663

Biomass and icosapentaenoic acid productivities from an outdoor batch culture of Phaeodactylum tricornutum UTEX 640 in an airlift tubular photobioreactor

  • E. Molina Grima
  • J. A. Sánchez Pérez
  • F. García Camacho
  • J. M. Fernández Sevilla
  • F. G. Acién Fernández
  • J. Urda Cardona
Biotechnology Original Paper

DOI: 10.1007/BF00171940

Cite this article as:
Molina Grima, E., Sánchez Pérez, J.A., García Camacho, F. et al. Appl Microbiol Biotechnol (1995) 42: 658. doi:10.1007/BF00171940

Abstract

An outdoor tubular photobioreactor, based on an external-loop airlift, has been used to cultivate the diatom Phaeodactylum tricornutum UTEX 640, rich in icosapentaenoic acid (EPA). The system was operated in batch mode. The specific growth rate in the exponential phase, averaged over the whole day, was 0.254 day−1 · A 25 gl−1 maximum biomass concentration was obtained by the end of the culture period. The dissolved oxygen generated never rose over 210% of air saturation nor was there impediment to biomass generation or inhibition of photosynthesis. Furthermore, around 2 × 1017 quanta cm−2 s−1 light intensity seemed to saturate the photosynthetic activity. Biomass losses during the night could be reduced to less than 5% by maintaining the culture at 16°C at night. The maximum concentration and productivity of EPA were 423 mgl−1 and 13 mgl−1 day−1, respectively, at the end of the culture, although a 21-mgl−1 day−1 net EPA generation rate was obtained at the linear growth phase.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • E. Molina Grima
    • 1
  • J. A. Sánchez Pérez
    • 1
  • F. García Camacho
    • 1
  • J. M. Fernández Sevilla
    • 1
  • F. G. Acién Fernández
    • 1
  • J. Urda Cardona
    • 1
  1. 1.Departamento de Ingeniería Química, Facultad de Ciencias ExperimentalesUniversidad de AlmeriaAlmeríaSpain