Applied Microbiology and Biotechnology

, Volume 40, Issue 2, pp 219–223

Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system

  • Toshiya Muranaka
  • Hideo Ohkawa
  • Yasuyuki Yamada
Biotechnology

DOI: 10.1007/BF00170370

Cite this article as:
Muranaka, T., Ohkawa, H. & Yamada, Y. Appl Microbiol Biotechnol (1993) 40: 219. doi:10.1007/BF00170370

Abstract

The scopolamine-releasing hairy root clone DL47-1 of Duboisia leichhardtii was cultured in an Amberlite XAD-2 column-combined bioreactor system for continuous production of scopolamine. The medium used was continuously exchanged during culture to maintain the electrical conductivity of the medium constant. After culturing the hairy roots in the system for 11 weeks, 0.5 g/l of scopolamine was obtained in the column. When the roots were cultures in the reactor system containing polyurethane foam or stainless-steel mesh to support the hairy roots, scopolamine recovery was increased. Thereafter, a two-stage culture, the first stage in the medium for hairy root growth and the second stage in the medium for scopolamine release, was carried out in this system by using a turbine-blade reactor with stainless-steel mesh as a support. Under these conditions, 1.3 g/l of scopolamine was recovered during 11 weeks of culture in the medium for scopolamine release. This bioreactor system seems applicable for the production of various plant metabolites by cultures of hairy roots.

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Toshiya Muranaka
    • 1
  • Hideo Ohkawa
    • 1
  • Yasuyuki Yamada
    • 2
  1. 1.Biotechnology Laboratory, Takarazuka Research CenterSumitomo Chemical Co., Ltd.Takarazuka HyogoJapan
  2. 2.Department of Agricultural Chemistry, Faculty of AgricultureKyoto UniversityKyotoJapan
  3. 3.Department of Plant Protection, Faculty of AgricultureKobe UniversityKobeJapan