Biodegradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil
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Degradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil was investigated. In experiments in which [9-14C]-phenanthrene was incubated with cultures of A. polychromogenes containing 150 mg phenanthrene/l it was shown that after 26 h of incubation 47.7% of the recovered radiolabelled carbon originally present was metabolized to 14CO2, 47.8% was recovered from the aqueous fraction, and 4.5% remained in the dichloromethane fraction. Increasing phenanthrene concentration in the culture medium resulted in improved growth and degradation rates, probably due to the higher amount of phenanthrene crystals in the medium. Shifting the temperature from 30°C to 35°C did not influence phenanthrene degradation significantly but inhibited cell division of A. polychromogenes. Medium supplementation with glucose led to stimulation of phenanthrene degradation at low amounts of glucose (0.45 g/l) whereas at higher concentrations (3 g/l) phenanthrene mineralization decreased.
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- Biodegradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil
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