Original Articles

Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 354, Issue 1, pp 80-85

First online:

Aconitine inhibits epileptiform activity in rat hippocampal slices

  • Angela AmeriAffiliated withDepartment of Pharmacy and Pharmacology of Natural Compounds, University of Ulm, Helmholtzstrasse 20
  • , Johannes GleitzAffiliated withDepartment of Pharmacy and Pharmacology of Natural Compounds, University of Ulm, Helmholtzstrasse 20
  • , Thies PetersAffiliated withDepartment of Pharmacy and Pharmacology of Natural Compounds, University of Ulm, Helmholtzstrasse 20

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Abstract

The effect of aconitine, an alkaloid neurotoxin known to bind at site 2 of the sodium channel, was investigated on epileptiform activity in hippocampal slices by use of extracellular recordings in CA1 pyramidal cell layer. Epileptiform activity was induced by bicuculline, picrotoxin, penicillin, pentylenetetrazol or by omission of magnesium from the bathing medium, respectively. In every case aconitine (0.1 and 1 gM) blocked the multiple population spikes representing the epileptiform activity. The onset of inhibition was shorter by use of an increased concentration of the epileptogenic drug. Epileptiform activity evoked by pentylenetetrazol and low magnesium was first increased by aconitine followed by a rapid inhibition, while the bicuculline-, picrotoxin-, and penicillin-induced epileptiform discharges were immediately abolished.

Key words

Aconitine Extracellular recordings Epileptiform activity Hippocampus