Inhibition of noradrenaline release from the sympathetic nerves of the human saphenous vein by presynaptic histamine H3 receptors

  • G. J. Molderings
  • G. Weißenborn
  • E. Schlicker
  • J. Likungu
  • M. Göthert
Article

DOI: 10.1007/BF00167569

Cite this article as:
Molderings, G.J., Weißenborn, G., Schlicker, E. et al. Naunyn-Schmiedeberg's Arch Pharmacol (1992) 346: 46. doi:10.1007/BF00167569

Summary

The human saphenous vein was used to examine whether presynaptic histamine receptors can modulate noradrenaline release and, if so, to determine their pharmacological characteristics. Strips of this blood vessel were incubated with [3H]noradrenaline and subsequently superfused with physiological salt solution containing desipramine and corticosterone. Electrically (2 Hz) evoked 3H overflow was inhibited by histamine and the H3 receptor agonist R-(−)-α-methylhistamine. Histamine-induced inhibition of electrically evoked tritium overflow was not affected by α2-adrenoceptor blockade by rauwolscine. S-(+)-α-methylhistamine (up to 10 μmol/l) as well as the histamine H1 and H2 receptor agonists 2-(2-thiazolyl)ethylamine (up to 3 μmol/l) and dimaprit (up to 30 μmol/l), respectively, were ineffective. The selective histamine H3 receptor antagonist thioperamide abolished the inhibitory effect of histamine. The histamine H2 and H1 receptor antagonists ranitidine and pheniramine, respectively, did not affect the histamine-induced inhibition of evoked tritium overflow. The present results are compatible with the suggestion that the sympathetic nerves of the human saphenous vein are endowed with inhibitory presynaptic histamine receptors of the H3 class.

Key words

Noradrenaline release Histamine H3 receptor Presynaptic receptors Human saphenous vein 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. J. Molderings
    • 1
  • G. Weißenborn
    • 1
  • E. Schlicker
    • 1
  • J. Likungu
    • 2
  • M. Göthert
    • 1
  1. 1.Institut für Pharmakologie und ToxikologieRheinische Friedrich-Wilhelms-Universität BonnBonn 1Federal Republic of Germany
  2. 2.Klinik für Herz- und GefäßchirurgieRheinische Friedrich-Wilhelms-Universität BonnBonn 1Federal Republic of Germany