Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 354, Issue 6, pp 717–724

Effects of secretogranin II-derived peptides on the release of neurotransmitters monitored in the basal ganglia of the rat with in vivo microdialysis

  • Z.-B. You
  • L. Terenius
  • A. Saria
  • R. Fischer-Colbrie
  • M. Goiny
  • M. Herrera-Marschitz
Original Article

DOI: 10.1007/BF00166897

Cite this article as:
You, ZB., Terenius, L., Saria, A. et al. Naunyn-Schmiedeberg's Arch Pharmacol (1996) 354: 717. doi:10.1007/BF00166897

Abstract

In vivo microdialysis was used to study the effect of secretogranin II-derived peptides on dynorphin B (Dyn B), dopamine, γ-aminobutyric acid (GABA), glutamate and aspartate release in the substantia nigra and neostriatum of halothane-anaesthesized rats.

In the substantia nigra, local infusion of secretoneurin (secretogranin II 154–186) (1–50 μM) increased, in a concentration-dependent manner, extracellular aspartate, glutamate, Dyn B, dopamine and GABA levels. The effect was particularly prominent on aspartate and glutamate levels which, following 50 μM of secretoneurin, were increased by >20 and >10 fold, respectively. However, the effect of secretoneurin on Dyn B release appeared to be more specific, since a significant increase (>2 fold) was already observed following 1 μM of secretoneurin. In the neostriatum, Dyn B, glutamate, aspartate and GABA levels were also increased by local secretoneurin infusion, but the effect was less prominent than in the substantia nigra. In the substantia nigra, only Dyn B levels were significantly increased following infusion of 10 μM of the secretoneurin-C terminal (secretoneurin-15C), whereas Dyn B and GABA levels were increased by the same concentration of the secretogranin II C terminus (YM). Only glutamate and aspartate levels were increased by local infusion of 10 μM of secretogranin II 133-151 (LF), a peptide adjacent to secretoneurin in the primary amino acid sequence. In the neostriatum, Dyn B and GABA levels were increased by 10 μM of secretoneurin-15C. Dyn B levels were also increased by 10 μM of YM, and glutamate and aspartate levels were increased by 10 μM of both YM and LF.

Thus, secretogranin 11-derived peptides affect extracellular levels of several putative neurotransmitter systems monitored in the basal ganglia of the rat with in vivo microdialysis. The effect of Dyn B appears to be specific and related to a physiological role of secretoneurin, since (i) it occurs in an area where secretoneurin-immunocytochemistry has been observed, (ii) is exerted at comparatively low concentrations, and (iii) is mimicked by secretoneurin-15C. The increases in excitatory amino acid levels produced by high concentrations of secretoneurin and other secretogranin II-derived peptides reflect, perhaps, a potential neurotoxicity produced by abnormal accumulation of these peptides.

Key words

Basal ganglia Neuropeptides Monoamines Amino acids Microdialysis Chromogranin C Secretogranin Rat 

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Z.-B. You
    • 1
    • 3
  • L. Terenius
    • 1
  • A. Saria
    • 2
  • R. Fischer-Colbrie
    • 2
  • M. Goiny
    • 3
  • M. Herrera-Marschitz
    • 3
  1. 1.Clinical NeurosciencesKarolinska InstituteStockholmSweden
  2. 2.Neurochemical Unit, Department of Psychiatry, University HospitalUniversity of InnsbruckInnsbruckAustria
  3. 3.Department of Physiology and PharmacologyKarolinska InstituteStockholmSweden

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