Applied Microbiology and Biotechnology

, Volume 41, Issue 1, pp 99–105

Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification

Authors

  • Akihiko Kondo
    • Applied Chemistry DepartmentKyushu Institute of Technology
  • Hiroko Kamura
    • Applied Chemistry DepartmentKyushu Institute of Technology
  • Ko Higashitani
    • Applied Chemistry DepartmentKyushu Institute of Technology
Applied Microbial and Cell Physiology

DOI: 10.1007/BF00166089

Cite this article as:
Kondo, A., Kamura, H. & Higashitani, K. Appl Microbiol Biotechnol (1994) 41: 99. doi:10.1007/BF00166089

Abstract

Ultrafine magnetite particles were prepared by a co-precipitation method. The poly-(styrene/N-isopropylacrylamide/methacrylic acid) latex particles containing ultrafine magnetite [magnetic P(St/NIPAM/MAA)] were prepared by two-step emulsifier-free emulsion polymerization. The minimum NaCl concentration for flocculation of these magnetic latex particles (critical flocculation concentration, CFC) decreased with increasing temperature. These temperature dependence of CFC, namely its thermo-sensitivity, originated from NIPAM. At a certain NaCl concentration, some of the magnetic latex particles showed reversible transition between flocculation and dispersion by controlling the temperature, and the thermo-flocculated magnetic latex particles were separated quickly in a magnetic field. Bovine serum albumin (BSA) was covalently immobilized onto the magnetic P(St/NIPAM/MAA) latex particles with high efficiency by the carbodiimide method. These thermo-sensitive magnetic immunomicrospheres were effective for the immunoaffinity purification of anti-BSA antibodies from antiserum.

Download to read the full article text

Copyright information

© Springer-Verlag 1994