Applied Microbiology and Biotechnology

, Volume 44, Issue 1, pp 210–217

13C-NMR study of lactate metabolism in Propionibacterium freudenreichii subsp. shermanii

  • D. B. Rolin
  • F. Girard
  • J. D. de Certaines
  • P. Boyaval
Applied Microbial and Cell Physiology

DOI: 10.1007/BF00164504

Cite this article as:
Rolin, D.B., Girard, F., de Certaines, J.D. et al. Appl Microbiol Biotechnol (1995) 44: 210. doi:10.1007/BF00164504


The adaptation to osmotic stress in Propionibacterium freudenreichii subsp. shermanii was investigated by using natural-abundance 13C nuclear magnetic resonance spectroscopy. Cells incubated either in a standard laboratory medium or in a medium designed to simulate the physicochemical conditions of Swiss-type cheese were found to accumulate different levels of osmotic-stress-protectant molecules. Proline, betaine, trehalose and glutamate were found simultaneously. Moreover, two types of polysaccharides were in evidence in this strain. Lactate catabolism was not mainly directed towards cell growth requirements and organic acid production but also towards biosynthesis of osmolytes requested for adaptation in a cheese environment. The possible involvement of such type of metabolite accumulation in the main cheese-ripening bacteria in Swiss-type cheeses is discussed.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • D. B. Rolin
    • 1
  • F. Girard
    • 2
  • J. D. de Certaines
    • 3
  • P. Boyaval
    • 4
  1. 1.Université de Rennes ILaboratoire de Biologie végétaleRennes CedexFrance
  2. 2.Institut Technique du GruyèreRennes CedexFrance
  3. 3.Université de Rennes ILaboratoire de RMN en Biologie et Médecine, Faculté de MédecineRennes CedexFrance
  4. 4.Laboratoire de Recherche en Technologie LaitièreInstitut National de la Recherche AgronomiqueRennes CedexFrance