Biotechnology Techniques

, Volume 8, Issue 3, pp 161–166

Optimization of amino acid supplements for heterologous protein secretion in Saccharomyces cerevisiae

  • K. D. Wittrup
  • V. Benig
Article

DOI: 10.1007/BF00161582

Cite this article as:
Wittrup, K.D. & Benig, V. Biotechnol Tech (1994) 8: 161. doi:10.1007/BF00161582
  • 243 Downloads

Summary

The two most commonly used amino acid media supplements do not improve the specific rate of secretion of Schizosaccharomyces pombe acid phosphatase in S. cerevisiae relative to unsupplemented Synthetic Dextrose (SD) media. New amino acid supplement formulations have been obtained which produce an 8-fold increase in total phosphatase secretion, and a 70% increase in specific phosphatase secretion.

Copyright information

© Science & Technology Letters 1994

Authors and Affiliations

  • K. D. Wittrup
    • 1
  • V. Benig
    • 1
  1. 1.Department of Chemical EngineeringUniversity of IllinoisUrbanaUSA

Personalised recommendations