Biotechnology Techniques

, Volume 10, Issue 7, pp 529–534

Estimation of the concentrations of cells, astaxanthin and glucose in a culture of Phaffia rhodozyma by near infrared reflectance spectroscopy

  • Yuichi Yamane
  • Takashi Mikami
  • Katsuya Higashida
  • Toshihide Kakizono
  • Naomichi Nishio
Article

DOI: 10.1007/BF00159519

Cite this article as:
Yamane, Y., Mikami, T., Higashida, K. et al. Biotechnol Tech (1996) 10: 529. doi:10.1007/BF00159519

Summary

Near infrared reflectance spectroscopy (NIR) was employed to estimate the concentrations of cells, astaxanthin and glucose in the culture broth of Phaffia rhodozyma. The culture broth (119 samples) was directly subjected for NIR analysis without any pretreatment. When the data obtained by NIR were compared with those obtained by conventional methods, high correlation coefficients were obtained: 0.98 for cells, 0.99 for astaxanthin and 0.94 for glucose. These results suggest that NIR analysis, which is very simple and requires only 3 to 5 mm for a sample, is applicable to monitor P. rhodozyma cultures.

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Yuichi Yamane
    • 1
  • Takashi Mikami
    • 2
  • Katsuya Higashida
    • 1
  • Toshihide Kakizono
    • 1
  • Naomichi Nishio
    • 1
  1. 1.Department of Fermentation Technology, Faculty of EngineeringHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Satake Co.Higashi-HiroshimaJapan