Biotechnology Letters

, Volume 18, Issue 8, pp 933–936

Adsorption of phage λ to Salmonella typhimurium lamB+ requires the presence of lipopolysaccharide in the outer membrane

  • M El Mzibri
  • C. Beudot
  • M. De Méo
  • M. Laget
  • H. Guiraud
  • G. Duménil
Article

DOI: 10.1007/BF00154624

Cite this article as:
El Mzibri, M., Beudot, C., De Méo, M. et al. Biotechnol Lett (1996) 18: 933. doi:10.1007/BF00154624
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Summary

Two S. typhimurium strains TA1534 (rfa+) and TA1538 (rfaE) were transformed with the lamB expression plasmid pAMH70. Transposition events with λplacMu55 hybrid phage were successful only with TA1534/pAMH70 strain. Using SDS-PAGE, the LamB protein was present in the total cell proteins but not in the outer membrane proteins of the TA1538/pAMH70 strain. The LamB protein must linked to the LPS of the outer membrane to allow adsorption of λ phage in S. typhimurium.

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • M El Mzibri
    • 1
  • C. Beudot
    • 1
  • M. De Méo
    • 1
  • M. Laget
    • 1
  • H. Guiraud
    • 1
  • G. Duménil
    • 1
  1. 1.Faculté de PharmacieLaboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)MarseilleFrance