Article

Biotechnology Letters

, Volume 18, Issue 8, pp 933-936

First online:

Adsorption of phage λ to Salmonella typhimurium lamB + requires the presence of lipopolysaccharide in the outer membrane

  • M El MzibriAffiliated withFaculté de Pharmacie, Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)
  • , C. BeudotAffiliated withFaculté de Pharmacie, Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)
  • , M. De MéoAffiliated withFaculté de Pharmacie, Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)
  • , M. LagetAffiliated withFaculté de Pharmacie, Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)
  • , H. GuiraudAffiliated withFaculté de Pharmacie, Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)
  • , G. DuménilAffiliated withFaculté de Pharmacie, Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784)

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Summary

Two S. typhimurium strains TA1534 (rfa +) and TA1538 (rfaE) were transformed with the lamB expression plasmid pAMH70. Transposition events with λplacMu55 hybrid phage were successful only with TA1534/pAMH70 strain. Using SDS-PAGE, the LamB protein was present in the total cell proteins but not in the outer membrane proteins of the TA1538/pAMH70 strain. The LamB protein must linked to the LPS of the outer membrane to allow adsorption of λ phage in S. typhimurium.