Article

Biotechnology Techniques

, Volume 8, Issue 6, pp 401-406

First online:

Production of endo-1,4-β-glucanase and xylanase by Trichoderma reesei immobilized on polyurethane foam

  • Reetta HaapalaAffiliated withLaboratory of Biotechnology and Food Engineering, Helsinki University of Technology
  • , Susan LinkoAffiliated withLaboratory of Biotechnology and Food Engineering, Helsinki University of Technology
  • , Elke ParkkinenAffiliated withAlko Ltd, Research Laboratories
  • , Pirkko SuominenAffiliated withAlko Ltd, Research Laboratories

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Summary

Endo-1,4-β-glucanase and xylanase were produced by Trichoderma reesei immobilized on polyurethane foam using lactose as the main carbon source. The most porous carrier was found to be the best of those tested. The nitrogen source and KH2PO4 concentration of the production medium had a marked effect on culture pH during the course of fermentation and, consequently, on xylanase activity. An increase in lactose concentration from 7 to 27 g/l resulted in an increase in endoglucanase activity (max. 730 U/ml), xylanase activity (max. 3350 U/ml) and filter paper activity (max. 3.0 FPU/ml).