Biotechnology Techniques

, Volume 8, Issue 6, pp 369–374

Stabilization of Candida lipase against acetaldehyde by adsorption onto celite

  • H. Kaga
  • B. Siegmund
  • E. Neufellner
  • K. Faber
  • F. Paltauf
Article

DOI: 10.1007/BF00154305

Cite this article as:
Kaga, H., Siegmund, B., Neufellner, E. et al. Biotechnol Tech (1994) 8: 369. doi:10.1007/BF00154305

Summary

Immobilization of Candida rugosa lipase (Amano AY-30) by adsorption onto Celite 545 led to a markedly improved performance of the enzyme: Firstly, the enzyme was almost completely resistant to deactivation by acetaldehyde, which is liberated as unavoidable by-product in acyl-transfer reactions with vinyl esters, and secondly, the enantioselectivity was enhanced up to three-fold.

Copyright information

© Chapman & Hall, London 1994

Authors and Affiliations

  • H. Kaga
    • 1
  • B. Siegmund
    • 1
  • E. Neufellner
    • 1
  • K. Faber
    • 1
  • F. Paltauf
    • 2
  1. 1.Institute of Organic ChemistryAustria
  2. 2.Institute of BiochemistryGraz University of TechnologyGrazAustria

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