Biotechnology Techniques

, Volume 5, Issue 1, pp 5–8

A highly efficient electroporation system for transformation of Bacillus licheniformis

  • Patrizia Brigidi
  • Edda De Rossi
  • Giovanna Riccardi
  • Diego Matteuzzi
Transformation

DOI: 10.1007/BF00152745

Cite this article as:
Brigidi, P., De Rossi, E., Riccardi, G. et al. Biotechnol Tech (1991) 5: 5. doi:10.1007/BF00152745

Summary

Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems.

Copyright information

© Science and Technology Letters 1991

Authors and Affiliations

  • Patrizia Brigidi
    • 1
  • Edda De Rossi
    • 2
  • Giovanna Riccardi
    • 2
  • Diego Matteuzzi
    • 1
  1. 1.Fermentation Chemistry and Industrial Microbiology, Department of Pharmaceutical SciencesUniversity of BolognaBolognaItaly
  2. 2.Department of Genetics and Microbiology “A. Buzzati Traverso”University of PaviaPaviaItaly