Biotechnology Techniques

, Volume 5, Issue 1, pp 5–8

A highly efficient electroporation system for transformation of Bacillus licheniformis

Authors

  • Patrizia Brigidi
    • Fermentation Chemistry and Industrial Microbiology, Department of Pharmaceutical SciencesUniversity of Bologna
  • Edda De Rossi
    • Department of Genetics and Microbiology “A. Buzzati Traverso”University of Pavia
  • Giovanna Riccardi
    • Department of Genetics and Microbiology “A. Buzzati Traverso”University of Pavia
  • Diego Matteuzzi
    • Fermentation Chemistry and Industrial Microbiology, Department of Pharmaceutical SciencesUniversity of Bologna
Transformation

DOI: 10.1007/BF00152745

Cite this article as:
Brigidi, P., De Rossi, E., Riccardi, G. et al. Biotechnol Tech (1991) 5: 5. doi:10.1007/BF00152745

Summary

Transformation of intact cells of Bacillus licheniformis 5A24 with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCP49 (7.1 kb) by electroporation resulted in 1.5 × 106, 1.2 × 106 , and 5.2 × 104 transformants per μg DNA, respectively. Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions. In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems.

Copyright information

© Science and Technology Letters 1991