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Agar, an alternative to agarose in analytical gel electrophoresis

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Biotechnology Techniques

Summary

Agarose is purified from the polysaccharide complex, agar. This purified fraction forms a neutral gel matrix that is commonly used in gel electrophoresis. We have found that for many routine uses, agar which is considerably less expensive than agarose can be used with satisfactory results.

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References

  • Aaij, C., and Borst, P. (1972) Biochim. Biophys. Acta 269, 192–200.

    Google Scholar 

  • Araki, C. (1937) J. Chem. Soc. Japan 58, 1338–1350.

    Google Scholar 

  • Araki C., and Arai K. (1956) Bull. Chem. Soc. Japan 29, 339–345.

    Google Scholar 

  • Hayward, G. S., and Smith, M. G. (1972) J. Mol. Biol. 63, 383–395.

    Google Scholar 

  • Helling, R. B., Goodman, H. M., and Boyer, H. W. (1974) J. Virol. 14, 1235–1244.

    Google Scholar 

  • Maniatis, T., Fritsch, E. F., and Sambrook, J. (1989) Molecular Cloning. A laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory, New York.

    Google Scholar 

  • McIndoe, W., and Munro, H. N. (1967) Biochim. Biophys. Acta 134, 458–460.

    Google Scholar 

  • Morrice, L. M., McLean, M. W., Long, W. F., and Williamson, F. B. (1983) Eur. J. Biochem. 133 673–684.

    Google Scholar 

  • Santos G. A., and Doty M. S. (1983) Botanica Marina 26, 31–34.

    Google Scholar 

  • Serwer P. (1983) Electrophoresis 4, 375–382.

    Google Scholar 

  • Sharp, P. A., Sugden, B., and Sambrook, J. (1973) Biochemistry 12, 3055–3063.

    Google Scholar 

  • Williams, B.L., and Wilson, K. (1979) Principles and Techniques of Practical Biochemistry. 2nd Edition, Edward Arnold Ltd., London.

    Google Scholar 

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Viljoen, C.D., Wingfield, B.D. & Wingfield, M.J. Agar, an alternative to agarose in analytical gel electrophoresis. Biotechnol Tech 7, 723–726 (1993). https://doi.org/10.1007/BF00152620

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  • DOI: https://doi.org/10.1007/BF00152620

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