Article

Cytotechnology

, Volume 1, Issue 2, pp 159-169

First online:

Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium

Egg yolk lipoprotein for serum-free growth of cells
  • Hiroki MurakamiAffiliated withInstitute of Food Science and Technology, 46-09, Department of Agriculture, Kyushu University
  • , Yuichi OkazakiAffiliated withInstitute of Food Science and Technology, 46-09, Department of Agriculture, Kyushu University
  • , Koji YamadaAffiliated withInstitute of Food Science and Technology, 46-09, Department of Agriculture, Kyushu University
  • , Hirohisa OmuraAffiliated withInstitute of Food Science and Technology, 46-09, Department of Agriculture, Kyushu University

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Abstract

Egg yolk lipoprotein promoted growth of a wide variety of mammalian cell lines, including plasma-cytomas and epithelial cell lines, in serum-free medium. The lipoprotein was active for cell growth when used with insulin, transferrin, ethanolamine and selenite. The most active lipoprotein fraction (YLP-pI7.5) was purified to give a single peak by chromatofocusing and gel filtration, and was homogeneous on a 0.35% agarose gel electrophoretogram. The lipoprotein was characterised as a very low density lipoprotein with a protein content of only 1.3%. This lipoprotein had an optimal concentration of 300 μg/ml (4 μg protein/ml). It was easily separable from proteinous molecules secreted into the serum-free medium by the cells, since it floated on the surface of the medium after addition of ammonium sulfate, to precipitate protein, and centrifugation. An associated structure of lipid and protein seemed to be still necessary for the lipoprotein to exhibit a growth promoting activity.

Key words

lipoprotein egg yolk serum-free medium cell growth monoclonal antibody ITES