Production of l-lactic acid with immobilized Lactobacillus delbrueckii
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- Stenroos, SL., Linko, YY. & Linko, P. Biotechnol Lett (1982) 4: 159. doi:10.1007/BF00144317
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Living Lactobacillus delbrueckii cells were entrapped in calcium alginate gel beads and employed both in recycle batch and continuous column reactors to produce l-lactic acid from glucose. The substrate contained l% (w/v) yeast extract as nutrient and 4.8% (w/v) solid calcium carbonate as buffer. The maxiumum lactic acid yield obtained was 97%, of which more than 90% was l-lactic acid. The biocatalyst activity half-life in continuous operation was about 100 d, and only about 10% of the activity was lost during intermittent storage of the bioreactor at +7°C for about 5 months.