Biotechnology Letters

, Volume 4, Issue 3, pp 159–164

Production of l-lactic acid with immobilized Lactobacillus delbrueckii

Authors

  • Sirkka-Liisa Stenroos
    • Department of ChemistryHelsinki University of Technology
  • Yu-Yen Linko
    • Department of ChemistryHelsinki University of Technology
  • P. Linko
    • Department of ChemistryHelsinki University of Technology
Article

DOI: 10.1007/BF00144317

Cite this article as:
Stenroos, S., Linko, Y. & Linko, P. Biotechnol Lett (1982) 4: 159. doi:10.1007/BF00144317

Summary

Living Lactobacillus delbrueckii cells were entrapped in calcium alginate gel beads and employed both in recycle batch and continuous column reactors to produce l-lactic acid from glucose. The substrate contained l% (w/v) yeast extract as nutrient and 4.8% (w/v) solid calcium carbonate as buffer. The maxiumum lactic acid yield obtained was 97%, of which more than 90% was l-lactic acid. The biocatalyst activity half-life in continuous operation was about 100 d, and only about 10% of the activity was lost during intermittent storage of the bioreactor at +7°C for about 5 months.

Copyright information

© Science and Technology Letters 1981