Clinical & Experimental Metastasis

, Volume 13, Issue 1, pp 49–56

Overexpression and localization of cathepsin B during the progression of human gliomas

Authors

  • Marupudi Sivaparvathi
    • Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer Center
  • Raymond Sawaya
    • Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer Center
  • Shang Wu Wang
    • Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer Center
  • Alan Rayford
    • Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer Center
  • Masaaki Yamamoto
    • Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer Center
  • Lance A. Liottat
    • Laboratory of PathologyNCI
  • Garth L. Nicolson
    • Department of Tumor BiologyThe University of Texas M. D. Anderson Cancer Center
  • Jasti S. Rao
    • Department of NeurosurgeryThe University of Texas M. D. Anderson Cancer Center
Research Papers

DOI: 10.1007/BF00144018

Cite this article as:
Sivaparvathi, M., Sawaya, R., Wu Wang, S. et al. Clin Exp Metast (1995) 13: 49. doi:10.1007/BF00144018

Abstract

Degradation of the extracellular matrix is a prerequisite for acquisition of the invasive phenotype. Several proteinases released by invading tumor cells appear to participate in the focal degradation of extracellular matrix proteins. Using an enzyme-linked immunosorbent assay, enzymatic assays, Western and Nothern blotting techniques, we determined whether increased levels of the cysteine protease cathepsin B correlated with the progression and invasion of human gliomas. The amount of cathepsin B activity and protein content were highest in glioblastomas, lower in anaplastic astrocytomas and lowest in normal brain tissue and low-grade gliomas. There were significantly higher amounts of Mr 25 000 and 26 000 bands in glioblastoma and anaplastic astrocytoma than in normal brain and low-grade glioma tissue extracts as determined by Western blotting with anti-cathepsin antibodies. In addition, cathepsin B transcripts were overexpressed in anaplastic astrocytoma (about two- to three-fold), in glioblastoma (about eight- to 10-fold), compared with normal brain tissue and low-grade glioma. Immunobistochemical staining for cathepsin B showed intense immunoreactivity in tumor and endothelial cells of glioblastomas and anaplastic astrocytomas but only weak immunoreactivity in low-grade glioma and normal brain tissues. Therefore, we conclude that cathepsin B expression is greatest in highly malignant astrocytomas, especially in glioblastomas, and is correlated with the malignant progression of astrocytomas.

Keywords

cysteine proteasesextracellular matrixglioblastoma multiformeinvasion

Copyright information

© Rapid Communications of Oxford Ltd 1995