Cytotechnology

, Volume 3, Issue 2, pp 149–156

Rapid in vitro transformation system for liver epithelial cells by iron chelate, Fe-NTA

Authors

  • Masayasu Yamada
    • Division of Cell BiologyShigei Medical Research Institute
  • Michiyasu Awai
    • Department of PathologyOkayama University Medical School
  • Tohru Okigaki
    • Division of Cell BiologyShigei Medical Research Institute
Original Research Papers

DOI: 10.1007/BF00143677

Cite this article as:
Yamada, M., Awai, M. & Okigaki, T. Cytotechnology (1990) 3: 149. doi:10.1007/BF00143677

Abstract

We have previously found toxic effects of iron chelate, Fe-NTA on cultured normal rat liver epithelial cells (RL34). In the present study, when RL34 cells were exposed to 50 μg/ml iron of Fe-NTA for 15 days, besides the expected cytolytic effects in most cells, the appearance of resistant cells was observed. The resistant cells showed drastic morphological transformation, grew in soft agar, and induced hepatocellular carcinomas when transplanted into syngeneic newborn rats in a short period of time. Since DNA instability in the transformed cells was ascertained by differential AO staining, it is suggested that DNA damage by Fe-NTA is of a critical importance for extremely rapid neoplastic transformation of normal epithelial cells.

Key words

DNA instabilityiron chelateFe-NTAliver epithelial cellsrapid in vitro transformation

Abbreviations

AO

acridine orange

DME medium

Dulbecco's modified Eagle's medium

FCS

fetal calf serum

Fe-HEDTA

ferric-N-(2-hydroxyethyl)ethylenediaminetriacetic acid

Fe-NTA

ferric-nitrilotriacetate

HEPES

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

PBS

Dulbecco's phosphate-buffered saline

Copyright information

© Kluwer Academic Publishers 1990