Biotechnology Letters

, Volume 17, Issue 7, pp 757–760

Increased ethanol production during growth of electric-field stimulated Kluyveromyces marxianus IMB3 during growth on lactose-containing media at 45°C

  • J. Simpson
  • D. Brady
  • A. Rollan
  • N. Barron
  • L. McHale
  • A. P. McHale
Article

DOI: 10.1007/BF00130364

Cite this article as:
Simpson, J., Brady, D., Rollan, A. et al. Biotechnol Lett (1995) 17: 757. doi:10.1007/BF00130364

Summary

Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional β-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • J. Simpson
    • 1
  • D. Brady
    • 1
  • A. Rollan
    • 1
  • N. Barron
    • 1
  • L. McHale
    • 1
  • A. P. McHale
    • 1
  1. 1.Biotechnology Research Group, School of Applied Biological and Chemical Sciences, University of UlsterColeraineNorthern Ireland