Increased ethanol production during growth of electric-field stimulated Kluyveromyces marxianus IMB3 during growth on lactose-containing media at 45°C
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- Simpson, J., Brady, D., Rollan, A. et al. Biotechnol Lett (1995) 17: 757. doi:10.1007/BF00130364
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Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional β-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.