Biotechnology Letters

, Volume 17, Issue 9, pp 985–988

The effects of phosphoric acid pretreatment on conversion of cellulose to ethanol at 45°C using the thermotolerant yeast Kluyveromyces marxianus IMB3

  • U. Nilsson
  • N. Barron
  • L. McHale
  • A. P. McHale
Article

DOI: 10.1007/BF00127439

Cite this article as:
Nilsson, U., Barron, N., McHale, L. et al. Biotechnol Lett (1995) 17: 985. doi:10.1007/BF00127439

Summary

Here we report on the effects of phosphoric acid pretreated cellulose as a substrate for ethanol production by K. marxianus IMB3 using simultaneous saccharification and fermentation systems at 45°C. With untreated, milled filter paper as substrate the maximum amount of ethanol produced was 25% of the maximum theoretical yield. After pre-treatment with 100% phosphoric acid, the yield increased to 42% of the maximum theoretical yield. When untreated microcrystalline cellulose was used as the fermentation substrate, yields of ethanol as 45°C amounted to 16% of the maximum theoretical yield whereas pretreatment of the substrate with phosphoric acid resulted in an increase in ethanol production to 69% of the maximum theoretical yield. This suggests that pretreatment of substrate with phosphoric acid would contribute to a reduction in the amount of exogenous enzyme needed.

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • U. Nilsson
    • 1
    • 2
  • N. Barron
    • 1
  • L. McHale
    • 1
  • A. P. McHale
    • 1
  1. 1.Biotechnology Research GroupSchool of Applied Biological and Chemical Sciences, University of UlsterColeraineNorthern Ireland
  2. 2.Dept. of BiotechnologyUniversity of LundSweden