Journal of Muscle Research & Cell Motility

, Volume 16, Issue 1, pp 35–43

Characterization of human skeletal muscle fibres according to the myosin heavy chains they express

  • Steven Ennion
  • José Sant' Ana Pereira
  • Anthony J. Sargeant
  • Archie Young
  • Geoffrey Goldspink
Papers

DOI: 10.1007/BF00125308

Cite this article as:
Ennion, S., Sant' Ana Pereira, J., Sargeant, A.J. et al. J Muscle Res Cell Motil (1995) 16: 35. doi:10.1007/BF00125308

Summary

Using a method of single muscle fibre analysis, we investigated the presence of RNA transcripts for various isoforms of the myosin heavy chain (MyoHC) gene in histochemically, immunohistochemically and electrophoretically characterized individual muscle fibres (n=65) from adult human vastus lateralis muscle. A cDNA clone isolated in this study was shown to contain the 3′ end of a previously uncharacterized human MyoHC gene which is expressed specifically in human fast IIA muscle fibres and we conclude that this clone contains part of the human fast IIA MyoHC gene. In all the fibres histochemically, immunohistochemically and electrophoretically characterized as containing the previously classified IIB MyoHC (n=23), it was shown that the human equivalent to the rat type IIX MyoHC gene is expressed. This observation was taken to suggest that the previously classified IIB muscle fibres in human muscle express a MyoHC isoform equivalent to the rat IIX, not the IIB, and would therefore be more accurately classified as IIX fibres.

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Steven Ennion
    • 1
    • 2
  • José Sant' Ana Pereira
    • 3
  • Anthony J. Sargeant
    • 3
  • Archie Young
    • 2
  • Geoffrey Goldspink
    • 1
  1. 1.Department of Anatomy and Developmental Biology Royal Free Hospital School of MedicineUniversity of LondonUK
  2. 2.Department of Geriatric Medicine, Royal Free Hospital School of MedicineUniversity of LondonUK
  3. 3.Department of Exercise and Muscle PhysiologyVrije UniversityAmsterdamThe Netherlands