Cell Biology and Toxicology

, Volume 5, Issue 2, pp 217–226

Use of cell cultures for predicting the biological effects of mycotoxins

  • Saida Robbana-Barnat
  • Christiane Lafarge-Frayssinet
  • Charles Frayssinet
Article

DOI: 10.1007/BF00122655

Cite this article as:
Robbana-Barnat, S., Lafarge-Frayssinet, C. & Frayssinet, C. Cell Biol Toxicol (1989) 5: 217. doi:10.1007/BF00122655

Abstract

The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of different origins. The response of several cell types to a number of well defined mycotoxins was obtained in three days. This approach allowed us to: demonstrate and quantify a toxic effect, define some organ specificity related to the preferential action on a particular cell type, and detect an immunosuppressive effect. The results indicate that the system can be used for toxicological screening and that it has a predictive value for the pathological effects of tested products.

Key words

Mycotoxins trichothecenes cell cultures specific toxicity 

Abbreviations

DAS

Diacetoxyscirpenol

DON

Deoxynivalenol

SV40

Simian virus 40

PHA

Phytohaemagglutinin

IC 50

Inhibiting concentration 50

LD 50

Lethal dose 50

DMSO

dimethylsulfoxide

Copyright information

© Princeton Scientific Publishing Co., Inc 1989

Authors and Affiliations

  • Saida Robbana-Barnat
    • 1
  • Christiane Lafarge-Frayssinet
    • 1
  • Charles Frayssinet
    • 1
  1. 1.Laboratoire de Pathologie Cellulaire de l'ER 278 (Reseau de toxicologie nutritionelle MRES)Institut de Recherches Scientifiques sur le CancerVillejuifFrance
  2. 2.Laboratoire de Pathologie CellulaireIRSCVillejuifFrance

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