Journal of Materials Science: Materials in Medicine

, Volume 5, Issue 9, pp 715–722

The effect of magnesium ion implantation into alumina upon the adhesion of human bone derived cells

  • C. R. Howlett
  • H. Zreioat
  • R. O'Dell
  • J. Noorman
  • P. Evans
  • B. A. Dalton
  • C. McFarland
  • J. G. Steele
Papers

DOI: 10.1007/BF00120363

Cite this article as:
Howlett, C.R., Zreioat, H., O'Dell, R. et al. J Mater Sci: Mater Med (1994) 5: 715. doi:10.1007/BF00120363

Our group is investigating the potential of modifying the surface atomic layers of biomaterials by ion beam implantation in order to stimulate adhesion of bone cells to these treated biomaterials. In this study alumina that had been implanted with magnesium ions (Mg)−(Al2O3), was compared to unmodified alumina (Al2O3) for the adhesion of cells cultured from explanted human bone. The attachment and spreading of cultured human bone derived cells onto (Mg)−(Al2O3) was significantly enhanced as compared to Al2O3. The role of adsorption of serum adhesive glycoproteins fibronectin (Fn) and vitronectin (Vn) in the adhesion of human bone derived cells to (Mg)−(Al2O3) was determined. The requirement for Fn or Vn for the attachment and spreading of bone-derived cells onto the Al2O3 and (Mg)−(Al2O3) surfaces was directly tested by selective removal of Fn or Vn from the serum prior to addition to the culture medium. The cell adhesion to both the alumina and the (Mg)−(Al2O3) surfaces in the presence of FBS was dependent upon serum Vn.

Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • C. R. Howlett
    • 1
  • H. Zreioat
    • 1
  • R. O'Dell
    • 2
  • J. Noorman
    • 3
  • P. Evans
    • 3
  • B. A. Dalton
    • 4
  • C. McFarland
    • 4
  • J. G. Steele
    • 4
  1. 1.School of PathologyUniversity of New South WalesKensingtonAustralia
  2. 2.Centre for Biomedical EngineeringUniversity of New South WalesKensingtonAustralia
  3. 3.Lucas Heights LaboratoriesANSTOLucas HeightsAustralia
  4. 4.Division of Biomolecular Engineering, Sydney LaboratoriesCSIRONorth RydeAustralia