, Volume 2, Issue 3-4, pp 259-260

Screening of a synthetic pentapeptide library composed of d-amino acids against fructose-1,6-biphosphate aldolase

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


A synthetic peptide library, theoretically composed of 537 824 d-amino acid pentapeptides anchored on polystyrene beads, was prepared with each bead bearing a single pentapeptide sequence. This library was screened for interaction with fructose-1,6-biphosphate aldolase of T. brucei labelled with biotin. Affinity beads that bound the enzyme were selected with streptavidin-coated magnetic beads. A total of 19 beads were isolated and individually subjected to Edman microsequence analysis. The corresponding peptide sequences were synthesized and evaluated for enzyme activity inhibition.