, Volume 56, Issue 3, pp 269-285

First online:

Application of cell and tissue culture and in vitro selection for disease resistance breeding — a review

  • R. W. van den BulkAffiliated withCentre for Plant Breeding and Reproduction Research (CPRO-DLO)

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Somaclonal variation, i.e. the variation induced by cell and tissue culture, offers an opportunity to broaden the genetic variation of crops. As a result of somaclonal variation a wide range of plant characteristics can be altered. However, the selection of agronomically important traits, e.g. disease resistance, has many limitations. The efficiency of selection can be increased by the application of in vitro selection procedures. Selection strategies that may be applied to obtain disease resistant somaclonal variants are described. Their merits and limitations, in relation to the efficiency of the procedures, the frequency of disease resistant variants and the genetics of the resistance obtained, are discussed.

Key words

disease resistance in vitro selection somaclonal variation tissue culture