Plant Molecular Biology

, Volume 31, Issue 3, pp 553–564

Primary structure and expression of plant homologues of animal and fungal thioredoxin-dependent peroxide reductases and bacterial alkyl hydroperoxide reductases

Authors

  • Margarete Baier
    • Lehrstuhl für Botanik IJulius-von-Sachs-Institut für Biowissenschaften
  • Karl-Josef Dietz
    • Lehrstuhl für Botanik IJulius-von-Sachs-Institut für Biowissenschaften
Regular Article

DOI: 10.1007/BF00042228

Cite this article as:
Baier, M. & Dietz, K. Plant Mol Biol (1996) 31: 553. doi:10.1007/BF00042228

Abstract

Higher plants express genes encoding peroxiredoxins of the two-cysteine type. This is concluded from the isolation of cDNAs from spinach (Spinacia oleracea) and barley (Hordeum vulgare cv. Gerbel) which are homologous to animal, fungal, and bacterial two-cysteine peroxiredoxins. Northern blot analysis indicated the presence of at least one corresponding gene in all angiosperms analyzed suggesting thatbas1 is a member of an ubiquitous gene family encoding a protein of fundamental importance in oxidative stress defense also in plants. In barley, expression increased upon application of methyl viologen but was not affected by ozone. mRNA levels increased during deetiolation in the light. Maximal abundance ofbas1 transcripts was observed in young developing shoot segments where cell division and elongation take place. Expression was insignificant in roots. The amount of bas1 protein was high in the leaf blade, particularly in etiolated plants, and did not respond to oxidative stress. bas1 protein was not detected in roots. From our data, we suggest that bas1 is an antioxidant enzyme particularly important in the developing shoot and photosynthesizing leaf.

Key words

cDNA-cloningexpressionHordeum vulgareleafperoxiredoxinshoot developmentSpinacia oleracea

Abbreviations

AhpC

C22 subunit of the alkyl hydroperoxide reductase

BSO

buthionine sulfoximine

DTT

dithiothreitol

EST

expressed sequence tag

TPx

thioredoxin-dependent peroxide reductase

SOD

CuZn superoxide dismutase

TR

thioredoxin reductase

Trx

thioredoxin

TSA

thiol-specific antioxidant

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Copyright information

© Kluwer Academic Publishers 1996