Plant Molecular Biology

, Volume 18, Issue 3, pp 581–584

Molecular identification of a soybean protein kinase gene family by using PCR

  • Xin -Hua Feng
  • Paul J. Bottino
  • Shain -dow Kung
Update Section Short Communication

DOI: 10.1007/BF00040673

Cite this article as:
Feng, X.H., Bottino, P.J. & Kung, S.. Plant Mol Biol (1992) 18: 581. doi:10.1007/BF00040673

Abstract

In this study we report identification of six members of a protein kinase gene family from soybean (Glycine max L.). Two fully degenerate oligonucleotide primers corresponding to two conserved motifs (DLK-PENV and GTHEYLAPE) in the catalytic domains of eukaryotic protein serine/threonine kinases were used in a polymerase chain reaction (PCR) to amplify soybean cDNA. Sequence analysis showed that 28 of the PCR sequences represented six different putative protein serine/threonine kinases. These results not only demonstrate that catalytic domains of protein kinases are highly conserved between plants and other eukaryotes but also suggest that there are multiple genes encoding protein kinases in plants.

Key words

gene family polymerase chain reaction protein kinases signal transduction soybean (Glycine max L.) 

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Xin -Hua Feng
    • 1
    • 2
  • Paul J. Bottino
    • 2
    • 3
  • Shain -dow Kung
    • 1
    • 2
  1. 1.Center for Agricultural BiotechnologyUniversity of MarylandCollege ParkUSA
  2. 2.Department of BotanyUniversity of MarylandCollege ParkUSA
  3. 3.Maryland Agricultural Experiment StationUniversity of MarylandCollege ParkUSA

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