Expression of desiccation-related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco
Update Section Short Communication
Received: 20 January 1992 Accepted: 10 June 1992 DOI:
Cite this article as: Iturriaga, G., Schneider, K., Salamini, F. et al. Plant Mol Biol (1992) 20: 555. doi:10.1007/BF00040614 Abstract
Three cDNAs encoding desiccation-induced proteins from the resurrection plant
Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3′-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests. Key words Chloroplast targeting desiccation tolerance drought-related proteins resurrection plant ( Craterostigma) transgenic tobacco References
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