Plant Molecular Biology

, Volume 24, Issue 1, pp 129–141

Subunit 6 of the Fo-ATP synthase complex from cytoplasmic male-sterile radish: RNA editing and NH2-terminal protein sequencing

Authors

  • Subbiah Krishnasamy
    • Department of ChemistryMiami University
  • Raymond A. Grant
    • Miami Valley LaboratoriesThe Procter and Gamble Co.
  • Christopher A. Makaroff
    • Department of ChemistryMiami University
Research Articles

DOI: 10.1007/BF00040580

Cite this article as:
Krishnasamy, S., Grant, R.A. & Makaroff, C.A. Plant Mol Biol (1994) 24: 129. doi:10.1007/BF00040580

Abstract

RNA editing and NH2-terminal processing of subunit 6 (atp6) of the mitochondrial Fo-ATPase complex has been investigated for the normal (fertile) and Ogura (male-sterile) radish cytoplasms to determine if previously identified differences between the Ogura atp6 locus and its normal radish counterpart are associated with cytoplasmic male sterility. Analysis of cDNA clones from five different sterile and fertile radish lines identified one C-to-U transition, which results in the replacement of a proline with a serine, in several of the lines. No editing of atp6 transcripts was observed in two lines, Scarlet Knight (normal radish) and sterile CrGC15 (Ogura radish). This is the first example of a naturally occurring plant mitochondrial gene that is not edited. The Ogura atp6 polypeptide is synthesized with a predicted NH2-terminal extension of 174 amino acids in contrast to the nine amino acid extension found in normal radish. In spite of the lack of similarity between the two extensions, NH2-terminal sequence analysis indicates that both polypeptides are processed to yield identical core proteins with a serine as the NH2-terminal residue. These results indicate that ATPase subunit 6 is synthesized normally in Ogura radish, and that it is unlikely that the atp6 locus is associated with Ogura cytoplasmic male sterility.

Key words

cytoplasmic male sterilityatp6RNA editingOgura cytoplasmmitochondrial DNA
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Copyright information

© Kluwer Academic Publishers 1994