Plant Cell, Tissue and Organ Culture

, Volume 43, Issue 3, pp 207–213

Use of starch-gelled medium for tissue culture of some fruit crops

  • Richard H. Zimmerman
  • S. V. Bhardwaj
  • Ingrid M. Fordham
Original Research Paper

DOI: 10.1007/BF00039946

Cite this article as:
Zimmerman, R.H., Bhardwaj, S.V. & Fordham, I.M. Plant Cell Tiss Organ Cult (1995) 43: 207. doi:10.1007/BF00039946
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Abstract

Six cultivars of apple and two of red raspberry consistently produced equal or significantly better shoot proliferation on modified Murashige and Skoog medium gelled with a mixture of corn starch and Gelrite than on the same medium gelled with agar. Two pear cultivars grown on starch-Gelrite medium produced hyperhydric shoots and almost no growth, but the addition of a polysaccharide hydric control (‘antivitrifying’) agent to the medium eliminated hyperhydricity. The resulting shoot proliferation equaled or exceeded that on the agar-gelled medium. The starch-Gelrite mixture is easy to prepare and gelling agent costs are only 10–15% of agar, or less if starch is purchased in bulk. Although the opaque gray-white medium makes it more difficult to detect internal contaminants, external contaminants are easily discerned.

Key words

antivitrificationhyperhydricitypolysaccharide

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Richard H. Zimmerman
    • 1
  • S. V. Bhardwaj
    • 1
  • Ingrid M. Fordham
    • 1
  1. 1.Fruit LaboratoryU.S. Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA
  2. 2.Department of BiotechnologyDr. Y.S. Parmar University of Horticulture and ForestrySOLANIndia