Plant Molecular Biology

, Volume 26, Issue 1, pp 487–493

Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development

  • Martha Matvienko
  • Karin Van De Sande
  • Wei-Cai Yang
  • Ab Van Kammen
  • Ton Bisseling
  • Henk Franssen
Short Communications

DOI: 10.1007/BF00039559

Cite this article as:
Matvienko, M., Van De Sande, K., Yang, WC. et al. Plant Mol Biol (1994) 26: 487. doi:10.1007/BF00039559

Abstract

A pea cDNA clone representing the homologue of the soybean pGmENOD40-1 was isolated and characterized. At the nucleotide level both clones share 55% homology. Strikingly, the homology between the polypeptides derived from the pea and soybean ENOD40 cDNA sequences is only 14%. Despite this low homology Southern analyses revealed that the isolated pea cDNA clone represents the single pea ENOD40. In situ hybridizations showed that at early stages of nodule development and in mature nodules the expression pattern of pea ENOD40 is comparable to that of soybean ENOD40. Although ENOD40 show similar expression patterns in these two nodules, it is questionable whether the putative polypeptides have a similar function, since the homology is very low.

Key words

peaearly nodulinindeterminate nodule

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Martha Matvienko
    • 1
    • 2
  • Karin Van De Sande
    • 1
  • Wei-Cai Yang
    • 1
  • Ab Van Kammen
    • 1
  • Ton Bisseling
    • 1
  • Henk Franssen
    • 1
  1. 1.Department of Molecular BiologyAgricultural UniversityWageningenNetherlands
  2. 2.Laboratory of BiotechnologyInstitute of Agricultural MicrobiologyPushkin-St.-PetersburgRussia