Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 2, pp 169–175

An evaluation of antibiotics for the elimination ofXanthomonas campestris pv.pelargonii (Brown) fromPelargonium x domesticum cv. ‘Grand Slam’ explants in vitro

  • Carol Barrett
  • Alan C. Cassells
Original Research Paper

DOI: 10.1007/BF00037716

Cite this article as:
Barrett, C. & Cassells, A.C. Plant Cell Tiss Organ Cult (1994) 36: 169. doi:10.1007/BF00037716

Abstract

A range of antibiotics was evaluated for activity againstXanthomonas campestris pv.pelargonii (Xep) on diagnostic sensitivity testing and plant tissue culture media. Many of the antibiotics showed reduced or no activity on the latter. Tetracycline and cefotaxime, chosen for further investigation, were screened for light stability under plant culture regimes. Tetracycline was inactivated in photosynthetic photon fluxes of 22 μmol m-2 s-1 and above. The minimum bacteriocidal concentration of cefotaxime was determined in bacteriological and plant tissue culture media. Cefotaxime was further tested for phytoxicity and ability to eliminate Xcp from deliberately infected explants. Cefotaxime was shown to eliminate contamination and stimulate the growth of the plant tissue cultures up 500 mg l-1.

Key words

bacterial contaminationcefotaximetissue culture

Abbreviations

DST

diagnostic sensitivity testing medium

MBC

minimum bacteriocidal concentration

TCM

half-strength Murashige & Skoog (1962) basal plant tissue culture medium

Xcp

Xanthomonas campestris pv.pelargonii

BA

benzyladenine

2,4-d

2,4-dichlorophenoxyacetic acid

OD

optical density

PPF

photosynthetic photon flux

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Carol Barrett
    • 1
  • Alan C. Cassells
    • 1
  1. 1.Department of Plant ScienceUniversity CollegeCorkIreland