Plant Molecular Biology

, Volume 17, Issue 4, pp 853–863

Evolution of the Rubisco operon from prokaryotes to algae: Structure and analysis of the rbcS gene of the brown alga Pylaiella littoralis

  • Nour-Eddine Assali
  • William F. Martin
  • Charles C. Sommerville
  • Susan Loiseaux-de Goër
Article

DOI: 10.1007/BF00037066

Cite this article as:
Assali, NE., Martin, W.F., Sommerville, C.C. et al. Plant Mol Biol (1991) 17: 853. doi:10.1007/BF00037066

Abstract

The rbcS gene coding for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) of the brown alga Pylaiella littoralis is located within the plastid genome and is transcribed as a single polycistronic mRNA with the gene for the large subunit of Rubisco, rbcL. The structure of the Rubisco operon from P. littoralis was determined. Molecular phylogenies for rbcS and rbcL with a wide range of prokaryotes and eukaryotes were constructed which are congruent with recent evidence for polyphyletic plastid origins. Both rbcL and rbcS of the β-purple bacterium Alcaligenes eutrophus clearly cluster with the rhodophyte and chromophyte proteins. The data suggest that the Rubisco operons of red algal and chromophytic plastids derive from β-purple eubacterial antecedents, rather than the cyanobacterial lineage of eubacteria from which other of their genes derive. This implies a lateral transfer of Rubisco genes from β-purple eubacterial ancestors to the cyanobacterial ancestor of rhodophyte and chromophyte plastids.

Key words

algaeendosymbiosisevolutionplastidial DNARubisco operon

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Nour-Eddine Assali
    • 1
  • William F. Martin
    • 2
  • Charles C. Sommerville
    • 3
  • Susan Loiseaux-de Goër
    • 3
  1. 1.Laboratoire de Biologie Moléculaire Végétale, CNRS, URA 57Université Joseph FourierGrenoble
  2. 2.Institut für GenetikTechnische Universität BraunschweigBraunschweigFRG
  3. 3.Station Biologique de RoscoffCNRS UPR 4601RoscoffFrance
  4. 4.Laboratoire de BiochimieI.A.V. Hassan IIRabatMorocco