Plant Molecular Biology

, Volume 27, Issue 5, pp 1053–1058

Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ-related alteration of NDK activities during rice seed germination (Oryza sativa L.)

  • Akira Yano
  • Masaaki Umeda
  • Hirofumi Uchimiya
Short Communication

DOI: 10.1007/BF00037032

Cite this article as:
Yano, A., Umeda, M. & Uchimiya, H. Plant Mol Biol (1995) 27: 1053. doi:10.1007/BF00037032

Abstract

The GST (glutathione S-transferase)-NDK (nucleoside diphosphate kinase) fusion protein was expressed in Escherichia coli. The GST-NDK protein was capable of transferring γ-phosphate from ATP to nucleoside diphosphates such as GDP, CDP, TDP and UDP. Western blot analysis using anti-NDK antibody indicated that NDK in endosperm gradually decreased during 36 h of imbibition. On the contrary, NDK in embryo increased during the same period. NDK activities in both tissues were in accord with these observations. Whereas the NDK protein in roots of rice seedlings during 7 days of imbibition remained constant, in shoots it declined after 5 days of imbibition. Thus, NDK may play a significant role in the cellular event modulated by adenylate energy charge level.

Key words

nucleoside diphosphate kinaseOryza sativa L.seed germination

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Akira Yano
    • 1
  • Masaaki Umeda
    • 1
  • Hirofumi Uchimiya
    • 1
  1. 1.Institute of Molecular and Cellular BiosciencesUniversity of TokyoTokyoJapan