Plant Molecular Biology

, Volume 27, Issue 5, pp 1043–1052

Gibberellin-regulated expression in oat aleurone cells of two kinases that show homology to MAP kinase and a ribosomal protein kinase

  • Alison K. Huttly
  • Andrew L. Phillips
Short Communication

DOI: 10.1007/BF00037031

Cite this article as:
Huttly, A.K. & Phillips, A.L. Plant Mol Biol (1995) 27: 1043. doi:10.1007/BF00037031

Abstract

cDNA fragments from ten different protein kinases expressed in Avena sativa aleurone cells were amplified from mRNA by RT-PCR with degenerate primers. These could be classified into five groups: Aspk1-3 showed homology to the Snf1-related protein kinases, Aspk4-5 to a wheat ABA up-regulated protein kinase, Aspk6-8 to the Ca-dependent, calmodulin-independent protein kinase family, Aspk9 encoded a MAP kinase and Aspk10 was closely related to a novel Arabidopsis ribosomal protein kinase. GA caused a rapid increase in transcripts hybridising to Aspk10, while inhibiting the dramatic accumulation of transcripts hybridising to Aspk9 that occurred in the absence of GA.

Key words

aleuroneAvena sativaGA regulationMAP kinasePCR amplificationprotein kinases

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Alison K. Huttly
    • 1
  • Andrew L. Phillips
    • 1
  1. 1.IACR-Long Ashton Research Station, Department of Agricultural SciencesUniversity of BristolBristolUK