Plant Cell, Tissue and Organ Culture

, Volume 34, Issue 2, pp 191–198

Propagation of Lirlodendron hybrids via somatic embryogenesis

  • Scott A. Merkle
  • Margaret T. Hoey
  • Beth A. Watson-Pauley
  • Scott E. Schlarbaum
Original Research Papers

DOI: 10.1007/BF00036101

Cite this article as:
Merkle, S.A., Hoey, M.T., Watson-Pauley, B.A. et al. Plant Cell Tiss Organ Cult (1993) 34: 191. doi:10.1007/BF00036101

Abstract

Propagation of hybrids between the Chinese tuliptree (Liriodendron chinense) and the North American yellow-poplar (Liriodendron tulipiferea) could be greatly accelerated with a highly productive somatic embryogenesis system. Flowers were collected from a single Chinese tuliptree and the anthers used for controlled pollinations of 4 yellow-poplar mother trees. Aggregates of samaras resulting from the pollinations were harvested 8 weeks post-pollination. Following surface disinfestation, samaras were dissected and embryos and endosperm were cultured together on a semisolid induction medium containing 9.0 μM 2,4-dichlorophenoxyacetic acid and 1.1 μM benzyladenine. Following 2–3 months on induction medium, an average of 15.6 percent of the explants produced either somatic embryos or proembryogenic masses. Compared to pure yellow-poplar embryogenic cultures, putative hybrid cultures tended not to maintain growth as proembryogenic masses while exposed to auxin, instead proliferating via repetitive embryogenesis as globular-stage embryos. Four to six weeks following transfer of globular embryos to basal medium, mature embryos were produced from the putative hybrid lines. Mature embryos germinated following transfer to basal medium lacking casein hydrolysate. Plantlets survived transfer to potting mix and acclimatization to greenhouse conditions. Verification of the hybrid nature of the embryogenic lines and somatic embryo-derived plantlets was accomplished by Southern hybridization analysis with a species-specific DNA marker.

Key words

yellow-poplar Chinese tuliptree micropropagation RFLP 

Abbreviations

BA

benzyladenine

2,4-d

2,4-dichlorophenoxyacetic acid

PEM

proembryogenic mass

CH

casein hydrolysate

RFLP

restricted fragment length polymorphism

CTAB

hexadecyltrimethylammonium bromide

TBE

Tris-borate-EDTA

SDS

sodium dodecylsufate

SSC

sodium citrate/chloride

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Scott A. Merkle
    • 1
  • Margaret T. Hoey
    • 2
  • Beth A. Watson-Pauley
    • 1
  • Scott E. Schlarbaum
    • 3
  1. 1.School of Forest ResourcesUniversity of GeorgiaAthensUSA
  2. 2.Genetics DepartmentUniversity of GeorgiaAthensUSA
  3. 3.Department of Forestry, Wildlife and FisheriesThe University of TennesseeKnoxvilleUSA

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